By: Heath Haggard and Dr. Wayne Shew
Birmingham’s
only National Allergy Bureau (NAB) pollen collection station is located on the
campus of Birmingham-Southern College, where it is run by Dr. Wayne Shew and
sponsored by Dr. Weily Soong and the Alabama Allergy and Asthma Center. The partnership between Birmingham-Southern
and Alabama Allergy and Asthma began in 2007 when Dr. Soong contacted Dr. Shew
to talk about the absence of a pollen station in Birmingham and the need for
establishing such a station that would be located within thirty miles of the
clinic’s practice. Dr. Shewfirst began
collecting pollen samples using a Rotorod collector, which was soon replaced
with the installation of a Burkard Volumetric Spore Trap. The pollen collecting station was certified
as an NAB site in the summer of 2010, and since that time the station has
maintained a consistent reporting of pollen counts to the National Allergy
Bureau. The station aids the Alabama
Allergy and Asthma Center by providing pollen and mold spore counts which
benefit the clinic’s patients, and also provides data useful for clinical
research and senior research opportunities to students at Birmingham-Southern
College.
The
pollen samples used at the station to generate counts are collected using a
Burkard Volumetric Spore Trap located on the roof of the Humanities building on
the campus. The Burkard spore trap
collects airborne particles on microscope slides coated with Dow Corning high
vacuum grease. Air is pulled through a 2
x 14 mm opening and impacts the microscope slide at a constant volume of 10
liters/minute. The air is sampled for a
24 hour period, the slide is removed, and the sample is mounted for microscopic
examination in glycerin jelly containing fuschin stain. Fuschin stain is selectively absorbed by the
pollen grains, which appear pink when viewed under a microscope at 400x. The Longitudinal Transverse Method, which
involves counting the number of specific pollen grains present in a horizontal
pass across the slide either one or two times, is used to count and identify
the pollen grains present on the slide.
The number of counted pollen grains can be converted to number of grains
per cubic meter of air using the following formula:
Pollen Grains/m3 of air= number
of grains counted
Vair sampled .
Volume of air sampled in the
formula described above is calculated using the following formula:
Vair (m3) = Field
Diameter (µm)
drum
rotation rate .
Pollen
counts generated at the Birmingham station can be accessed through the Alabama
Allergy and Asthma Center’s website, http://www.alabamaallergy.com/, or through
the American Academy of Asthma and Immunology’s National Allergy Bureau, http://www.aaaai.org/global/nab-pollen-counts.aspx.
This was written by Heath Haggard of Alabama
Allergy & Asthma Center and there subsidiary Clinical Research Center
of Alabama in conjunction with Dr. Wayne Shew at Birmingham Southern College.
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